sheep polyclonal anti gfp antibody  (Novus Biologicals)

Journal: bioRxiv

Article Title: Chronic but not acute morphine exposure reversibly impairs spike generation and repetitive firing in a functionally distinct subpopulation of orexin neurons

doi: 10.1101/2025.03.20.644444

Figure Lengend Snippet: A. Schematic of experimental cohorts, injection timing and tissue preparation. B. Low power (4x objective) and C. higher power (40x objective) epifluorescence images of EGFP-positive neurons within the orexin neuron field. Anti-orexin-A immunofluorescence (red), anti-GFP immunofluorescence (green). D. Efficacy (percent orexin immunopositive neurons also expressing GFP immunofluorescence) and specificity (percent GFP immunopositive neurons also expressing orexin immunofluorescence). E ., F . Brightfield low power (E) and higher power (F) of GFP-immunopositive neurons visualized with VIP. Yellow polygon delineates the soma outline (F) used for measurements in G-J. G . GFP-immunopositive soma area per mouse from saline-treated (S; blue symbols) and morphine-treated (M; red symbols) mice. H. Histogram of all measured GFP-immunopositive soma sizes from each treatment group (S, blue, n = 356 cells; M, red, n = 440 cells). I . Cumulative fraction of soma sizes from each treatment group (S, blue symbols; M, red symbols; Kolmogorov-Smirnov test p < 0.0001. Abbreviations: f, fornix; S, saline-treated; M, morphine-treated. Numbers are reported as mean ± sem.

Article Snippet: For identification of GFP, sections were incubated with sheep polyclonal anti-GFP antibody (1:1,000, Novus Biologicals, #NB100-62622, lot#1710).

Techniques: Injection, Immunofluorescence, Expressing, Saline

Journal: iScience

Article Title: Acquisition of neural fate by combination of BMP blockade and chromatin modification

doi: 10.1016/j.isci.2023.107887

Figure Lengend Snippet:

Article Snippet: Sheep polyclonal anti-Green Fluorescent Protein (GFP) (1:1000) , Bio-Rad , Cat#4745-1051; RRID: AB_619712.

Techniques: Recombinant, Knock-Out, Isolation, Purification, Imaging, Western Blot, Software, CRISPR

Journal: Developmental cell

Article Title: Rapid dynamics of signal-dependent transcriptional repression by Capicua

doi: 10.1016/j.devcel.2020.02.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fly lines generated in this study are listed in the and are available at the laboratory upon request. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Sheep polyclonal anti-GFP Bio-Rad Cat#4745-1051; RRID: AB_619712 Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) Invitrogen Cat#A-11015; RRID: AB_141362 Chemicals, Peptides, and Recombinant Proteins DAPI fluorescent DNA stain ThermoFisher Scientific Cat#D1306 RRID: AB_2629482 Critical Commercial Assays Protein G Dynabeads for immunoprecipitation Invitrogen Cat#10003D Proteinase K NEB Cat# P8107S NEBNext Ultra II DNA Library Prep Kit for Illumina NEB Cat#E7645S NEBNext Multiplex Oligos for Illumina Kit NEB Cat#E7335S Zymogen ChIP DNA Clean and Concentrator Kit Zymogen Cat#D5205 PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25741 Deposited Data Raw ChIP-seq data and list of Cic targets This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE130584","term_id":"130584"}} GSE130584 Analysis scripts This paper https://github.com/keenansh/SEK_Cic_ChIP_Analysis .

Techniques: Recombinant, Staining, Immunoprecipitation, Multiplex Assay, SYBR Green Assay, Software